Composite

Part:BBa_K2622046

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


SurA composite

Chaperone involved in the correct folding and assembly of outer membrane proteins, such as OmpA, OmpF and LamB. Recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins. Tagged with His-6x, under control of T7 promoter and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 326
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1434
    Illegal PstI site found at 326
    Illegal NotI site found at 1373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1075
    Illegal XhoI site found at 1382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 326
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 326
    Illegal AgeI site found at 1186
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None