Composite
Part:BBa_K2622046
Designed by: Auksė Gaižauskaitė Group: iGEM18_Vilnius-Lithuania (2018-10-10)
SurA composite
Chaperone involved in the correct folding and assembly of outer membrane proteins, such as OmpA, OmpF and LamB. Recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins. Tagged with His-6x, under control of T7 promoter and terminator.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 326
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1434
Illegal PstI site found at 326
Illegal NotI site found at 1373 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1075
Illegal XhoI site found at 1382 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 326
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 326
Illegal AgeI site found at 1186 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |